Composite
Gun4+

Part:BBa_K1080013

Designed by: Macquarie University   Group: iGEM13_Macquarie_Australia   (2013-09-22)

GUN4 (+ Ptac Promoter)

Tetrapyrrole-binding protein - In Arabidopsis, GUN4 (Genomes uncoupled 4) is required for the functioning of the plastid mediated repression of nuclear transcription that is involved in controlling the levels of magnesium- protoporphyrin IX. GUN4 binds the product and substrate of Mg-chelatase, an enzyme that produces Mg-Proto, and activates Mg-chelatase. GUN4 is thought to participates in plastid-to-nucleus signaling by regulating magnesium-protoporphyrin IX synthesis or trafficking. This composite part contains the promoter BBa_K864400. The Ptac promoter is a functional hybrid promoter, derived from the trp and lac promoters, that are regulated by trp and lac [1].

[1] Proc. Natl. Acad. Sci. USA, Vol. 80, pp. 21-25

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 788
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 473
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None